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Image Search Results
Journal: Nature Communications
Article Title: Membrane lipid nanodomains modulate HCN pacemaker channels in nociceptor DRG neurons
doi: 10.1038/s41467-024-54053-z
Figure Lengend Snippet: a , b Representative phasor plots showing FRET between L10-CFP and hHCN2-YFP expressed in small DRG neurons before and after 10 nM paclitaxel treatment. c Summary data of the effects of the β-CD and paclitaxel on the FRET between L10-CFP and hHCN2-YFP. Data shown are mean ± s.e.m., n = 7 for control and β-CD conditions, n = 8 for paclitaxel conditions, ** p = 1e-6 for β-CD and ** p = 4e-6 for paclitaxel compared with the control. One-way ANOVA (no adjustment) was used. d Cartoon showing the design of the FRET sites in the VSD of human HCN2 channels and the representative phasor plot showing FRET between donor AF-488 labeled to the HCN2-L323TAG site and the FRET acceptor AF-555 labeled to the HCN2-T240TAA site. e Representative phasor plot of the same FRET pair as in panel ( d ) following acute 5 mM β-cyclodextrin treatment. f Summary data of the effect of the β-CD on the FRET efficiency of hHCN2-L323TAG/T240TAA channels. Data shown are mean ± s.e.m., n = 5 cells for the control and n = 4 cells after β-CD, ** p = 0.002 using two-sided student’s t -test. g Representative phasor plot of the same FRET pair as in panels ( d ) and ( e ), following acute 5 mM β-cyclodextrin treatment with 120 mM KCl in the bath. h Summary data of the effect of the β-CD on the FRET efficiency as shown in panel ( g ). Data shown are mean ± s.e.m., n = 7 cells for the control and n = 6 cells with β-CD, p = 0.054 using two-sided student’s t -test.
Article Snippet:
Techniques: Control, Labeling